Wetlab Techniques | TanBio R and D Solution | Vallam | Thanjavur | Tamilnadu

S.No.	BASIC	ADVANCED
1.	Adsorption chromatography	Affinity chromatography
2.	Thin layer chromatography	Hydrophobic interactions
3.	Ion exchange chromatography	High performance liquid chromatography (analytical and preparative)
4.	Gel filtration chromatography	Ultra performance liquid chromatography
5.	-	Gas chromatography mass spectrometry
6.	-	Liquid chromatography mass spectrometry

S.No.	CONTENT
1.	Phytochemical screening
2.	Amino acid screening
3.	Estimation of Carbohydrate
4.	Estimation of Lipid
5.	Estimation of Vitamins
6.	Ammonia
7.	Calcium
8.	Magnesium
9.	Nitrate
10.	Nitrite
11.	Chloride
12.	Phosphate
13.	Sulphate
14.	Methylene Blue Reductase Test
15.	Catalase And Coagulase Test
16.	Estimation Of Soil Mineral Content
17.	Lecithinase Test
18.	Carbohydrate Fermentation Test
19.	Triple Sugar Iron Agar
20.	Urease Test
21.	Voges-Proskauer Test
22.	Litmus Milk Test
23.	DPPH - Antioxidant test

S.No.	CONTENT
1.	Construction of Maltose Standard Curve by DNS Method
2.	Construction of Protein Standard Curve using Folin’s Lowry Method
3.	Effect of substrate concentration on enzyme kinetics
4.	Effect of temperature on enzyme kinetics
5.	Estimation of blood glucose by Glucose oxidase method
6.	Estimation of saponification value of fats/ oils
7.	Isoelectric precipitation of proteins: Casein from milk
8.	Qualitative Analysis of Amino Acid
9.	Qualitative analysis of carbohydrates
10.	Quantitative Estimation of Amino Acids by Ninhydrin
11.	Separation of Amino Acids by Thin Layer Chromatography
12.	Titration Curves of Amino acids
13.	Estimation of Blood Urea by Enzymatic Berthelot method

S.No.	CONTENT
1.	Microbial degradation of cellulose
2.	Isolation of coilforms from sewage
3.	Analysis of TDS of effluent
4.	Effect of high salt concentration on microbial growth

S.No.	CONTENT
1.	Moisture
2.	Fat
3.	Oil
4.	Food
5.	Water
6.	Fiber
7.	Ash
8.	Carbohydrate
9.	Energy
10.	Minerals(Na,K,Mg,Ca,Fe,Mn,Zn,Se,Cu,S,Mo,P)
11.	Heavy metals analysis (Hg, Pb,As, and Cd)

S.No.	CONTENT
1.	Preparation of culture media
2.	Sterilization
3.	Cleaning and sterilization of glass wars
4.	Aseptic technique and culture inoculation
5.	Serial dilution technique
	Pure Culture Techniques
6.	Pour plate method
7.	Spread plate technique
8.	Streak plate
9.	Cultural variation of bacteria
10.	Morphological variation of bacteria
	Staining Techniques
11.	Smear preparation and fixation
12.	Simple Staining
13.	Staining
14.	Gram staining
15.	Negative Staining
16.	Spore Staining
17.	Acid fast Staining
18.	Silver Staining
19.	Motility test
20.	Cultivation of anaerobes’
21.	Preservation microorganism

S.No.	CONTENT
1.	Antibody generation,
2.	Detection of molecules using ELISA,
3.	RIA
4.	Western blot
5.	Immunoprecipitation,
6.	Luocytometry
7.	Immunofluorescence microscopy,
8.	FISH
9.	GISH
10.	Widal Test

S.No.	CONTENT
1.	Measurement of growth and growth curve
2.	Effect of growth
3.	Effect of Temperature on growth
4.	Effect of salinity on growth
5.	Effect of sunlight radian on growth
6.	Effect of Disinfectant (phenol test)

S.No.	CONTENT
1.	Isolation of bacteria from soil
2.	Isolation of fungi from soil
3.	Isolation of actinomyceles from soil

S.No.	CONTENT
1.	Visualization of cells and sub cellular components by light microscopy
2.	Scanning electron microscopy
3.	Transmission electron microscopy
4.	Atomic force microscopy
5.	Inverted microscopy
6.	Florescence microscopy

S.No.	CONTENT
1.	Assessment of Dented Proteins by SDS PAGE Electrophoresis
2.	Assessment of DNA by Agarose Gel Electrophoresis
3.	Extraction of Bacteriophage DNA from Large Scale Cultures Using Proteinase K and SDS
4.	Extraction of DNA from Agarose gel
5.	Extraction of DNA from Fish Fins
6.	Hot Shot Method of DNA Extraction
7.	Isolation of RNA
8.	Ligation ( Using T4 DNA Ligase)
9.	Plasmid Curing
10.	Plasmid Isolation (Mini prep) phage, cosmid, BAC and YAC vectors.
11.	Polymerase Chain Reaction (PCR).
12.	Preparation of Buffer stocks (TBE, TE and TAE)
13.	Preparation of Competent Cell (Calcium Chloride Treatment)
14.	Restriction Digestion
15.	Transformation of the Host Cells
16.	RFLP, RAPD and AFLP techniques

S.No.	CONTENT
1.	Preformulation Studies For Active Pharmaceutical Ingredient (Api) And Granules
2.	Determination Of Particle Size Distribution Of Granules By Sieve Analysis Method
3.	Determination Of Particle Size Distribution By Optical Microscopy
4.	Estimation Of Optimum Concentration Of Various Glidants For The Flow Of Granules Using Angle Of Repose By Funnel Method
5.	Effect Of Particle Size Reductions On Surface Area Of Powder
6.	Evaluation Of Quality Control Parameters Of Tablets And Capsules
7.	Study Of Extract Of Protein Binding

S.No.	CONTENT
1.	Formulation And Evaluation Of Paraceutical Suspension
2.	Formulation And Evaluation Of Multiple Emulsion
3.	Formulation And Evaluation Of Neutralizing Capacity Of Antacid Gel
4.	Formulation And Evaluation Of Cephalexin Dry Syrup
5.	Preparation And In-Vitro Evaluation Of In –Situ Gels Of Ciprofloxacin For Ocular Delivery
6.	Formulation And Evaluation Of Sustained Release Tablets –Matrix And Reservoir Type
7.	Formulation And Evaluation Of Microscope